Publications

Cover Articles

(* indicates co-first authors / # indicates corresponding authors) 

[21] Unravelling the Maturation Pathway of a Eukaryotic Virus through Cryo-EM.
Castells-Graells, R.#, Hesketh, E.L., Matsui, T., Johnson, J.E., Ranson, N.A., Lawson, D.M., Lomonossoff, G.P.#
BioRxiv (2024) doi.org/10.1101/2024.08.31.610394

[20]  X-ray crystal structure of a designed rigidified imaging scaffold in the ligand-free conformation.
Agdanowski, M., Castells-Graells, R., Sawaya, M., Cascio, D., Yeates, T.O., Arbing, M.A.
Acta Cryst Section F (2024) doi.org/10.1107/S2053230X2400414X

Selected as the cover image for the journal!

[19]  Designed fluorescent protein cages as fiducial markers for targeted cell imaging.
Gee, M., Atai, K., Coller, H.A., Yeates, T.O., Castells-Graells, R.#
BioRxiv (2024) doi.org/10.1101/2024.02.28.582585

[18]  Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion.
Gladkov, N., Scott, E.A., Meador, K., Lee, E.J., Laganowsky, A.D., Yeates, T.O.#, Castells-Graells, R.#
Protein Science (2024) doi.org/10.1002/pro.4973

Selected as the cover image for the journal!

[17]  Making topological protein links using enzymatic reactions.
Castells-Graells, R, Yeates, T.O.
National Science Review (2024) doi.org/10.1093/nsr/nwae071

[16]  A suite of designed protein cages using machine learning algorithms and protein fragment-based protocols.
Meador, K.*, Castells-Graells, R.*, Aguirre, R., Sawaya, M.R., Arbing, M.A., Sherman, T., Senarathne, C., Yeates, T.O.
Structure (2024) doi.org/10.1016/j.str.2024.02.017

Selected as the cover image for the journal!

[15]  The Design of Self-Assembling Protein Cages and Other Reticular Protein Materials. In: Chemistry Challenges of the 21st Century.
Castells-Graells, R., Laniado, J., Meador, K., Yeates, T.O.
World Scientific (2024) doi.org/10.1142/9789811282324_0022

[14]  Cryo-EM structure determination of small therapeutic protein targets at 3 Å resolution using a rigid imaging scaffold.
Castells-Graells, R., Meador, K., Arbing, M.A., Sawaya, M.R., Gee, M., Cascio, D., Gleave, E., Debreczeni, J.É., Breed, J., Leopold, K., Patel, A., Jahagirdar, D., Lyons, B., Subramaniam, S., Phillips, C., Yeates, T.O.
Proceedings of the National Academy of Sciences (2023) doi.org/10.1073/pnas.2305494120

In the news: 

UCLA Newsroom: "UCLA-led team develops key improvement to Nobel Prize-winning technology"

Chemistry World: "Cryo-EM scaffold supports imaging of small proteins previously tricky to study"

[13]  Design of beta-2 microglobulin adsorbent protein nanoparticles.
Miller, J.E.*, Castells-Graells, R.*, Arbing, M.A.*, Munoz, A., Jiang, Y.X., Espinoza, C.T., Nguyen, B., Moroz, P., Yeates, T.O.
Biomolecules (2023) doi.org/10.3390/biom13071122

[12]  Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.
Castells-Graells, R., Ribeiro, J., Domitrovic, T., Hesketh, E.L., Scarff, C.A., Johnson, J.E., Ranson, N.A., Lawson, D.M., & Lomonossoff, G.P.
Communications biology (2021) doi.org/10.1038/s42003-021-02134-w

In the news:

EurekAlert: "Virus infection cycle revealed in dynamic detail"

[11]  A replicating viral vector greatly enhances accumulation of helical virus-like particles in plants.
Thuenemann, E.C., Byrne, M.J., Peyret, H., Saunders, K., Castells-Graells, R., Ferriol, I., Santoni, M., Steele, J., Ranson, N.A., Avesani, L., Lopez-Moya, J.J., & Lomonossoff, G.P.
Viruses (2021) doi.org/10.3390/v13050885

[10]  Plant-based production can result in covalent cross-linking of proteins.
Castells-Graells, R., & Lomonossoff, G. P.
Plant biotechnology journal (2021) doi.org/10.1111/pbi.13598

[9]  Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis.
Schreier, T.B., Fahy, B., David, L.C., Siddiqui, H., Castells-Graells, R., & Smith, A.M.
The Plant Journal (2021) doi.org/10.1111/tpj.15246

[8]  Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process.
Petre, B., Contreras, M.P., Bozkurt, T.O., Schattat, M.H., Sklenar, J., Schornack, S., Abd-El-Haliem, A., Castells-Graells, R., Lozano-Durán, R., Dagdas, Y. F., Menke, F., Jones, A., Vossen, J. H., Robatzek, S., Kamoun, S., & Win, J.
The Plant Cell (2021) doi.org/10.1093/plcell/koab069

[7]  Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization.
Johnson, J.E., Domitrovic, T., Matsui, T., Castells-Graells, R., & Lomonossoff, G.P.
Archives of virology (2021) doi.org/10.1007/s00705-021-05007-z

[6]  High stability of plant-expressed virus-like particles of an insect virus in artificial gastric and intestinal fluids.
Berardi, A., Castells-Graells, R., & Lomonossoff, G.P.
European journal of pharmaceutics and biopharmaceutics (2020) doi.org/10.1016/j.ejpb.2020.08.012

[5]  OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis.
Del Cerro, P., Ayala-García, P., Buzón, P., Castells-Graells, R., López-Baena, F.J., Ollero, F.J., & Pérez-Montaño, F.
Applied and environmental microbiology (2020) doi.org/10.1128/AEM.01297-20 

[4]  NRC4 Gene Cluster Is Not Essential for Bacterial Flagellin-Triggered Immunity.
Wu, C.H., Adachi, H., De la Concepcion, J.C., Castells-Graells, R., Nekrasov, V., & Kamoun, S.
Plant physiology (2020) doi.org/10.1104/pp.19.00859

[3]  Production of mosaic turnip crinkle virus-like particles derived by coinfiltration of wild-type and modified forms of virus coat protein in plants.
Castells-Graells R., Lomonossoff G.P., Saunders K.
Methods in Molecular Biology (2018) doi.org/10.1007/978-1-4939-7808-3_1

[2]  Synthetic plant virology for nanobiotechnology and nanomedicine.
Steele, J.F.C., Peyret, H., Saunders, K., Castells-Graells, R., Marsian, J., Meshcheriakova, Y., Lomonossoff, G.P.
WIREs Nanomedicine & Nanobiotechnology (2017) doi.org/10.1002/wnan.1447

[1]  The impact of transposable elements in the evolution of plant genomes: From selfish elements to key players.
Contreras, B., Vives, C., Castells, R., Casacuberta, J.M.
Evolutionary Biology: Biodiversification from Genotype to Phenotype (2015) doi.org/10.1007/978-3-319-19932-0_6